Flow Cytometry Optical Filter

Flow cytometry filters are essential for selectively detecting and analyzing fluorescence signals emitted by different cellular components and markers.

Coligh, a leading manufacturer in the field, provides an extensive range of optical filters specifically designed for flow cytometers. These flow cytometry filters filters are crafted to optimize the detection and analysis of fluorescent signals across a broad spectrum of wavelengths. Below is an overview of Coligh’s filter offerings and their applications:

Blue Laser (488 nm) Flow cytometry Filters

1. 450/50: Designed for detecting blue fluorescence, such as DAPI. This filter isolates light around 450 nm with a 50 nm bandwidth, ideal for high-resolution detection of specific blue fluorophores.
2. 530/30: Used for green fluorescence detection, such as FITC and GFP. This filter captures light around 530 nm with a narrow 30 nm bandwidth, providing precise measurements for green fluorescent markers.
3. 575/25: Optimized for yellow fluorescence detection, including PE. It captures light around 575 nm with a 25 nm bandwidth, allowing for accurate assessment of yellow fluorescent signals.
4. 630/22: Intended for red fluorescence detection, such as PerCP. This filter isolates light around 630 nm with a 22 nm bandwidth, useful for distinguishing red fluorescent markers.
5. 488/10: Designed for narrow-bandwidth detection around 488 nm, suitable for fine resolution in green fluorescence applications.

Red Laser (633 nm or 635 nm) Flow cytometry Filters

1. 650/25: Suitable for detecting far-red fluorescence, such as PE-Cy5. This filter captures light around 650 nm with a 25 nm bandwidth, essential for analyzing far-red fluorescent signals.
2. 670/30: Designed for red fluorescence detection, such as APC. It isolates light around 670 nm with a 30 nm bandwidth, perfect for detecting red fluorescent markers.
3. 710/50: Ideal for near-infrared fluorescence, such as APC-Cy7. This filter captures light around 710 nm with a 50 nm bandwidth, facilitating the analysis of near-infrared fluorescent signals.
4. 638/10: Optimized for narrow-bandwidth detection around 638 nm, used for precise measurement of far-red fluorescence.

Violet Laser (405 nm) Flow cytometry Filters

1. 450/50: Used for detecting blue fluorescence, such as V450. This filter isolates light around 450 nm with a 50 nm bandwidth, suitable for high-resolution detection in the violet spectrum.
2. 525/50: Optimized for detecting cyan fluorescence, including Pacific Blue. It captures light around 525 nm with a 50 nm bandwidth, providing accurate measurements for cyan fluorescent markers.
3. 610/20: Designed for detecting blue-purple fluorescence, such as Dazzle Blue. This filter captures light around 610 nm with a 20 nm bandwidth, ideal for analyzing specific blue-purple fluorescent signals.
4. 460/55: Designed for detecting blue fluorescence, such as Hoechst. This filter captures light around 460 nm with a 55 nm bandwidth, suitable for a broad range of blue fluorescent markers.

Near-Infrared Laser (800 nm) Flow cytometry Filters

1. 750/50: Suitable for near-infrared fluorescence, such as Alexa Fluor 750. This filter captures light around 750 nm with a 50 nm bandwidth, facilitating the detection of near-infrared fluorescent markers.
2. 780/60: Optimized for detecting near-infrared fluorescence, including APC-Cy7. It isolates light around 780 nm with a 60 nm bandwidth, providing precise measurements for near-infrared fluorescent signals.

Other Flow cytometry Filters Configurations

1. 490/30: Designed for detecting green fluorescence, such as CFSE. This filter captures light around 490 nm with a 30 nm bandwidth, suitable for analyzing green fluorescent markers.
2. 525/50: Used for detecting green fluorescence, including GFP. It isolates light around 525 nm with a 50 nm bandwidth, ideal for accurate detection of green fluorescent signals.
3. 535/40: Optimized for detecting green fluorescence, such as FITC. This filter captures light around 535 nm with a 40 nm bandwidth, enhancing the measurement of green fluorescent signals.
4. 560/40: Optimized for red fluorescence detection, such as R-PE. This filter captures light around 560 nm with a 40 nm bandwidth, facilitating the analysis of red fluorescent markers.
5. 580/20: Used for detecting yellow fluorescence, such as PE. This filter isolates light around 580 nm with a 20 nm bandwidth, suitable for precise measurement of yellow fluorescent signals.
6. 665/20: Designed for detecting deep red fluorescence, such as PerCP-Cy5.5. This filter captures light around 665 nm with a 20 nm bandwidth, ideal for accurate deep red fluorescence detection.

Coligh’s filters are meticulously engineered to enhance the performance of flow cytometers by providing precise wavelength isolation and minimizing interference. These filters are integral to accurately sorting and analyzing cells based on their fluorescence characteristics, thereby improving the reliability and quality of cytometric data.