Coligh’s Standard 488nm Optical Bandpass Filter FWHM 50nm

The 488nm optical bandpass filter is engineered to transmit visible light centered at 488nm with a Full Width at Half Maximum (FWHM) of 50nm. This filter is ideal for applications requiring moderate bandwidth and high light transmission, such as fluorescence microscopy, flow cytometry, and other biomedical imaging systems.

Spectrum Specification:

• Type: VIS (Visible) Bandpass Filter
• Center Wavelength (CWL): 488nm ± 5nm
• Angle of Incidence: 0° ± 5°
• FWHM: 50nm ± 5nm
• Blocking Range: 300nm – 1100nm
• Blocking Depth: OD2, OD3, OD4, OD5, OD6, or higher
• Transmission: > 90% peak
• Thickness: 1.0mm, 1.1mm
• Sizes: Square or round (e.g., φ8mm, φ10mm, φ12.5mm, φ25.4mm, φ50mm) with optional mounting

Custom Options:

• Bandwidth: Custom FWHM options available upon request. 10nm, 15nm, 20nm, 25nm, 30nm, 35nm, 40nm, 45nm, 60nm
• Angle of Incidence: Adjustable based on specific application needs.
• Blocking Depth: Customizable to any OD level required.
• Sizes: Available in square or round formats, with maximum dimensions up to 150mm x 150mm.
• Material Thickness: Adjustable according to project requirements.
• Transmission & Blocking Range: Customizable, with blocking range options extending from 300nm to 2000nm.

Its application:

• Fluorescence Microscopy: Used to excite specific fluorophores like GFP (Green Fluorescent Protein) for clear and accurate imaging of cells and tissues.
• Flow Cytometry: Helps isolate the 488nm wavelength for accurate cell sorting and analysis, commonly used in immunology and cancer research.
• Confocal Laser Scanning Microscopy: Enhances image clarity by transmitting only the desired blue-green light for detailed 3D visualization of biological samples.
• Biomedical Imaging: Provides precise wavelength selection in diagnostic imaging devices, improving the accuracy of measurements and detections.
• Spectroscopy & Analytical Instruments: Used in spectrophotometers for analyzing biological samples, chemical reactions, or environmental samples with high sensitivity.
• Laser Systems: Compatible with 488nm lasers, making it ideal for research and industrial laser applications, including laser-induced fluorescence.
• Optogenetics: Enables precise activation of light-sensitive proteins in neuroscience research, aiding in the study of brain function.
• DNA Sequencing: Facilitates accurate detection of fluorescent tags in automated sequencing systems.