Fluorescence instrument

Fluorescence instruments are commonly used in scientific research, medical diagnostics, and industrial applications to detect and measure fluorescent signals from samples. Optical filters are a critical component of fluorescence instruments. They help to isolate the desired wavelengths of light emitted by the sample and filter out unwanted background noise.

In fluorescence instruments, optical filters are typically used in the excitation and emission pathways. The excitation filter is placed in front of the light source to select the wavelength range that excites the fluorophores in the sample. The emission filter is placed in front of the detector to block the excitation light and select the range of wavelengths emitted by the fluorophores.

The choice of optical filters in fluorescence instruments depends on the specific application and fluorophores used. Some common types of optical filters used in fluorescence instruments include bandpass filters, longpass filters, shortpass filters, dichroic mirrors, and notch filters. These filters can be designed to have specific wavelength ranges, transmission spectra, and optical densities to meet the requirements of the instrument and application.

Here are some of the common instruments of optical filters in fluorescence:

Fluorescence microscopy: Fluorescence microscopy is a widely used technique in biological research, which utilizes fluorescent dyes to label specific molecules or structures in cells. Optical filters are used to selectively pass the emission wavelength of the fluorophore while blocking the excitation light and other unwanted wavelengths. This enables researchers to visualize and study the labeled structures with high specificity and sensitivity.

Flow cytometry: Flow cytometry is a technique that allows the analysis of individual cells or particles in a sample. Fluorescent dyes or proteins are often used to label specific cell types or intracellular structures, and optical filters are used to selectively detect the fluorescence signal. This enables the analysis of cell populations and the identification of specific cell types based on their fluorescence properties.

Fluorescence spectroscopy: Fluorescence spectroscopy is a technique that measures the fluorescence emission of a sample when excited with a specific wavelength of light. Optical filters are used to select the appropriate excitation and emission wavelengths for the fluorophore of interest. This enables the measurement of the fluorescence properties of molecules in solution, such as their fluorescence lifetime and intensity.

Fluorescence-based assays: Fluorescence-based assays are commonly used in drug discovery, environmental monitoring, and clinical diagnostics. Optical filters are used to selectively detect the fluorescence signal from the labeled analyte while blocking other fluorescent or non-fluorescent components in the sample. This enables the detection and quantification of specific molecules or analytes in the sample with high sensitivity and specificity.

Coligh’s catalog fluorescence filters set and custom fluorescence filters will meet all your demands on various fluorescence applications.