HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set is high performance fluorescence filter set specially design for the detection of HEX、VIC yellow fluorophore in PCR (qPCR).
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set Product Discription
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set is high performance fluorescence filter set specially design for the detection of HEX、VIC yellow fluorophore in PCR (qPCR). It consists of exitation filter 535/25nm, emission filter 560/20nm and 550nm dichroic filter. Coligh manufactures and custom PCR filter set.
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set Product Features
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set is manufactured using ion-assisted hard coating technology, enabling the extraction of faint fluorescent signals from background noise during fluorescence detection. It features the following characteristics:
- Specifically designed for detecting yellow fluorophores HEX and VIC, with an excitation filter center wavelength of 535nm and an emission filter center wavelength of 560nm. This perfectly covers the excitation and emission ranges of HEX and VIC fluorescent dyes, ensuring maximum excitation efficiency and capturing the most emitted light.
- We achieve this through multi-layer hard coating on optical glass. This durable process ensures the hard coating bonds firmly to the glass substrate while maintaining stable optical performance, guaranteeing minimal signal decay over extended periods.
- All our filters maintain center wavelengths within +/-2nm tolerance, enabling precise wavelength control.
- Both the bandpass filter and dichroic mirror in the kit feature steep edge transitions, minimizing crosstalk.
- Both excitation and emission filters achieve transmission rates exceeding 90%, ensuring thorough dye excitation and emission. Concurrently, cutoff depths surpass OD6, minimizing background noise.
- The dichroic mirror efficiently reflects excitation light while transmitting emission light, fundamentally preventing color crosstalk.
- We currently offer multiple standard sizes and custom dimensions to meet client requirements.
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set Spectrum Curve
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set Technical Specification
| Item | Specification |
|---|---|
| Product Name | HEX/VIC/TET PCR Filter Set |
| Excitation Filter (EX) | 544/15nm |
| Emission Filter (EM) | 585/20nm |
| Dichroic Mirror (DM) | 565nm |
| Optical Density | OD>=6 |
| Transmission | > 90% |
| Compatible Fluorophores | HEX, VIC, TET, other yellow-green dyes |
| Substrate Material | Optical glass or fused silica |
| Diameter | Standard: 25 mm / 12.5 mm (customizable) |
| Thickness | 1.0mm or custom |
| Recommended Applications | Real-time PCR, qPCR, DNA/RNA detection |
Coligh PCR Fluorescence Filter Custom Capability
| Exitation filter wavelength range | 300–750 nm or custom |
|---|---|
| Emission filter wavelength range | 400–850 nm or custom |
| Blocking depth | OD5 OD6 |
| FWHM | 6–50 nm |
| Dichroic filter | Custom reflection and emission wavelength range |
| Size | 5×5 mm – 50×50 mm or φ5–50 mm |
| Substrate | Fused silica、B270 and other optical glass |
HEX/VIC Yellow PCR Fluorescent Bandpass Filter Set Application
- Real-time quantitative PCR: The HEX/VIC filter kit selectively excites fluorescent dyes such as HEX and VIC for fluorescence detection, monitoring DNA amplification during the PCR reaction and providing real-time, accurate DNA quantification data.
- Multiplex qPCR: During qPCR, filters such as FAM, HEX/VIC, and Cy5 are cyclically switched to acquire signals from each channel. The HEX/VIC fluorescence filter kit can be used as part of fluorescence detection to construct triple or quadruple detection methods.
