380nm UV OD6 Steep Edge Cut-off Filter Overview

The 380nm UV OD6 Steep Edge Cut-off Filter achieves high-efficiency UV light transmission in the 380nm range and maintains an ultra-high OD6 cutoff depth and steep transition edge in the 200-1100nm range. It is suitable for applications such as fluorescence microscopy, UV fluorescence imaging, flow cytometry, fluorescence spectroscopy, UV fluorescence immunoassay, POCT, and UV light sources.

Coligh, as a manufacturer of UV edge filters and fluorescence filters, provides design, prototyping, customization, and mass production services for UV fluorescence excitation filters. We use UV-grade fused silica substrates, featuring low autofluorescence, low deep UV absorption, and good stability. Our hard dielectric multilayer film design ensures high transmittance within the passband and deep cutoff within the cutoff range, resulting in stable and durable filters.

We can customize optical parameters such as cutoff wavelength, transition bandwidth, and passband range based on the fluorescent dye, excitation wavelength, emission wavelength, microscope optical path, and detector type.

380nm UV OD6 Steep Edge Cut-off Filter Key Features

1. Achieving an OD6 cutoff depth up to 1100nm in the long-wavelength range completely eliminates background stray light and signal crosstalk.
2. An extremely steep and sharp edge transition design at 380nm ensures pure excitation light.
3. UV-grade fused silica exhibits low autofluorescence and low absorption in the deep ultraviolet region, making it ideal for weak fluorescence detection.
4. The IAD multilayer dielectric hard film provides the filter with excellent spectral performance while maintaining durability.

380nm UV OD6 Steep Edge Cut-off Filter Spectrum

380nm UV OD6 Steep Edge Cut-off Filter Specification

• 50% cut off：380+/-5nm
• 390-1100nm@OD6 blocking depth
• Tp>=85%@330-386nm

Stock List Of Optical Shortpass Filter

Coligh Customize Capability Of Shortpass Fluorescence Exitation Filter

As a professional optical filter manufacturer, we specialize in solving the core challenges of fluorescence imaging and analytical systems: eliminating excitation leakage, suppressing background autofluorescence, and achieving sharp spectral separation. We operate complete, independent production lines ranging from UV-grade substrate processing to high-precision thin-film coating, allowing us to tailor cut-on/cut-off wavelengths, steep transition slopes, OD blocking depths, dimensions, shapes, and incident angles (AOI) to your exact fluorescence optical path.

380nm UV OD6 Steep Edge Cut-off Filter Applications

Fluorescence Microscopy UV Excitation Channel

In fluorescence microscopy, dyes such as DAPI, Hoechst, Alexa Fluor 350, and Cascade Blue require UV excitation, with emission light typically in the visible light band. The excitation light intensity is much higher than the emission fluorescence; if excitation light leaks into the detector channel, it will mask the weak fluorescence signal, reducing the signal-to-noise ratio and image contrast. A 380 nm steep-edge cutoff filter is used in the excitation channel to allow UV excitation light below 380 nm to pass through, while a deep OD6 cutoff blocks visible emission fluorescence and stray light above 380 nm. The steep-edge design shortens the transition region and improves excitation/emission separation.

Flow Cytometry UV Excitation Channel

In flow cytometry, UV lasers or UV lamps are used to excite specific fluorescent dyes. The fluorescence signal is weak, and the detection speed is fast. Excitation light leakage and ambient light can reduce detection sensitivity and resolution. Flow cytometry typically uses multiple lasers and multiple fluorescence channels, and cross-coloration between channels is one of the main challenges. A 380 nm OD6 steep-edge cutoff filter can be used in the UV excitation channel to deeply suppress visible emission and stray light, improving signal purity and detection accuracy.

Fluorescence Spectroscopy and UV Fluorescence Detection

Fluorescence spectrometers require the separation of UV excitation light and visible emission fluorescence. An OD6 steep-edge cutoff filter can deeply suppress excitation light leakage, improving spectral purity and detection limits. In weak fluorescence spectroscopy, trace detection, and time-resolved fluorescence spectroscopy, background noise directly affects detection capability; a deep cutoff filter can effectively reduce background noise. The steep-edge design reduces the influence of the transition region on the target spectrum.