395nm Longpass Dichroic Mirror  Overview

A 395nm longpass dichroic mirror is an optical filter that reflects 330-380nm and transmits 400-850nm at an incidence angle of 45°. It is primarily used in fluorescence optical paths to separate excitation light from emission fluorescence. Typically mounted at a 45° incidence angle in a fluorescence cube or optical path, it reflects the excitation light onto the sample while allowing the emitted fluorescence to be transmitted to the detector. It is commonly used in fluorescence optical paths for ultraviolet excitation dyes such as DAPI, Hoechst, Alexa Fluor 350, and AMCA. It is mainly used in fluorescence microscopes, flow cytometers, fluorescence imaging, confocal microscopes, multiphoton imaging, fluorescence spectrometers, ultraviolet/visible spectrophotometers, and scientific instruments.

Coligh customs and manufactures single-band and multi-band dichroic mirrors for clients.

Customization of cutoff wavelength, reflection band, transmission band, transition width, size, and thickness is available based on the client’s fluorescent dye, excitation wavelength, emission wavelength, optical path angle, and mounting method. Circular, square, and mounting ring customizations are supported.

395nm Longpass Dichroic Mirror Key Features

1. The 395nm long-pass design reflects short-wave ultraviolet light and transmits long-wave visible fluorescence.
2. 45° incident angle optimization.
3. High reflectivity in the 330-380nm band (R>=99%).
4. High transmittance in the 400-850nm band (T>=90%).

395nm Longpass Dichroic Mirror Spectrum

395nm UV Dichroic Beamsplitter Specification

• AOI: 45 deg
• 330-380nm@R>=99%
• 400-850nm@T>=90%

Stock List Of Dichroic Filter

Coligh Customize Capability Of Dichroic Filter

395nm Longpass Dichroic Filter Applications

Fluorescence Microscopy

In fluorescence microscopy, a 395 nm long-pass dichroic mirror is mounted at 45° to reflect ultraviolet excitation light below 395 nm to the sample, while allowing visible fluorescence above 395 nm to pass through to the detector, achieving efficient separation. This improves the fluorescence signal-to-noise ratio and weak signal recognition capability, making it suitable for nuclear imaging, cell imaging, tissue imaging, and multiplex fluorescence imaging.

Flow Cytometry

In flow cytometry, weak fluorescence signals and fast detection speeds mean that excitation light leakage and ambient light can reduce detection sensitivity and resolution. A 395 nm long-pass dichroic mirror can be used to separate ultraviolet excitation light and visible fluorescence, improving signal purity and detection accuracy.

Fluorescence Imaging

In in vivo imaging and fluorescence imaging, tissue autofluorescence and ambient light can reduce the signal-to-noise ratio. A 395 nm long-pass dichroic mirror can effectively separate ultraviolet excitation light and visible fluorescence, reducing excitation light leakage and short-wavelength autofluorescence.